/10 Report a question What's wrong with this question? You cannot submit an empty report. Please add some details. 12345678910 Chapter 9 : Biotechnology - Principles and Processes This Quiz Contains a set of 10 Questions. The Passing Cut-off is 60%. To start the Quiz, click on the Start Button. All the Best! 1 / 10 The enzyme that seals the nicks between sugar-phosphate backbones is Topoisomerase DNA helicase DNA ligase DNA polymerase 2 / 10 The antibiotic tetracycline is used in pBR322 to Stop translation Activate promoters Identify recombinant cells Enhance replication 3 / 10 The restriction enzyme EcoRI recognises the sequence 5′–GATATC–3′ 5′–CTGCAG–3′ 5′–GAATTC–3′ 5′–GGATCC–3′ 4 / 10 DNA ligase is an enzyme that Cuts DNA at specific sites Removes RNA primers Joins cut DNA fragments Synthesizes RNA strands 5 / 10 In genetic engineering, plasmids are preferred as vectors because they Lack antibiotic resistance Are linear molecules Are single-stranded Can replicate independently 6 / 10 The device used to maintain optimum growth conditions for large-scale culture is Fermenter Bioreactor Chromatograph Centrifuge 7 / 10 In PCR, each cycle of amplification results in Doubling of DNA quantity No change in quantity Linear increase of DNA Halving of DNA quantity 8 / 10 The basic principle of gel electrophoresis is Diffusion of solutes in water Movement of charged molecules in an electric field Separation by solubility Centrifugal force separation 9 / 10 The term “vector” in genetic engineering refers to DNA molecule used to transfer genes Restriction enzyme Viral infection agent Host cell 10 / 10 The blue-white selection in cloning vectors is based on the activity of Restriction enzyme RNA polymerase β-galactosidase enzyme DNA ligase Your score is 0% Restart quiz
